| Annotated protein: | 5-hydroxytryptamine receptor 2A (5-HT-2) (5-HT-2A) (Serotonin receptor 2A). Gene symbol: HTR2A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P14842 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HTR2A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | neocortex |
| Annotated paper: | Miner LA, et al. "Ultrastructural localization of serotonin2A receptors in the middle layers of the rat prelimbic prefrontal cortex" Neuroscience. 2003;116(1):107-17 PMID:12535944 |
| Figure(s): | Fig 2, Fig 3, Fig 4 |
| Annotation description: | Using immunocytochemistry and electron microscopy, the referenced study established that in the rat prelimbic prefrontal cortex, most 5-HT2a receptors are located within postsynaptic structures, predominantly on proximal and distal dendritic shafts. 5-HT2a receptor immunoreactivity was observed in 325 identifiable structures. Of these, 73% were postsynaptic profiles that were composed of either dendritic shafts (58%) or dendritic spine heads and necks (42%). 21/11/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | Generation of antibody and validation of the specificity using immunoblots and immunocytochemistry (antibody name JB-124) @ PMID:9402563 |
| SynGO annotation ID: | 1480 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |