| Annotated protein: | Metabotropic glutamate receptor 1 (mGluR1). Gene symbol: GRM1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P23385 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRM1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus cerebellum |
| Annotated paper: | Baude A, et al. "The metabotropic glutamate receptor (mGluR1 alpha) is concentrated at perisynaptic membrane of neuronal subpopulations as detected by immunogold reaction" Neuron. 1993 Oct;11(4):771-87 PMID:8104433 |
| Figure(s): | Fig 1-7; Table 1 |
| Annotation description: | Location: Peri- and Extrasynaptic location of mGluR1a Detected by a Particulate lmmunomarker Technique: They used a high resolution immunometal method that produces silver particles on a gold core, in three different immunocytochemical techniques. These included preembedding experiments on thick sections, reaction on frozen ultrathin sections, and postembedding reaction on ultrathin sections obtained from Lowicryl-embedded tissue. The use of these combined techniques allowed the precise localization of mGluR1a at the membrane of immunoreactive cells. Results were obtained from the hippocampus (Figure 4) and cerebellum (Figure 5; Figure 6; Figure 7) Results: 1) The most striking result of the present study is that immunoreactivity for mGluR1a is not found in the main body of the postsynaptic density, but is concentrated in the postsynaptic membrane at the periphery of synaptic junctions. 2) Their results demonstrate a high concentration of receptor close to the transmitter release site, but not in the main body of the synaptic junction. An identical localization of the receptor was found with three different techniques. 3) An antiserum to mGluR1a labeled a 160 kd protein in immunoblots of membranes derived from rat brain or cells transfected with mGluR1a. lmmunoreactivity for mGluR1 a was present in discrete subpopulations of neurons. (Table 1) 21/11/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 1472 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |