| Annotated protein: | Protein kinase C delta type (EC 2.7.11.13) (nPKC-delta) [Cleaved into: Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit (Sphingosine-dependent protein kinase-1) (SDK1)]. Gene symbol: PRKCD. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P09215 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRKCD |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | thalamus |
| Annotated paper: | Merchenthaler I, et al. "Light and electron microscopic immunocytochemical localization of PKC delta immunoreactivity in the rat central nervous system" J Comp Neurol. 1993 Oct 15;336(3):378-99 PMID:8263228 |
| Figure(s): | Figure 17E |
| Annotation description: | Using immuno- electron microscopy it is shown that PKC delta is present in the postsynaptic cytosol. No knockout control is provided. As control, the antibody was pre-absorbed against the immunizing peptide. 10/3/2017 Pim: -Fig17E is guess this EM picture is difficult to asses (by me). Authors conclude: "Axon terminal (A) establishing multiple synaptic specializations (arrowheads) with a PKCG-i (PKC) dendrite (De)". Dendritic labeling close to synapses (arrow) is considered as "presynaptic". |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 146 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |