| Annotated protein: | Glutamate receptor ionotropic, kainate 4 (GluK4). Gene symbol: GRIK4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BMF5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIK4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Darstein M, et al. "Distribution of kainate receptor subunits at hippocampal mossy fiber synapses" J Neurosci. 2003 Sep 3;23(22):8013-9 PMID:12954862 |
| Figure(s): | Fig. 4, 5 |
| Annotation description: | KA1 kainate receptor 8/9/2017 Pim Fig.4: "Ultrastructural localization of kainate receptor subunits at mossy fiber synapses" - Literal: "Localization of gold labeling was common in the presynaptic mossy terminals (m) as well as in postsynaptic spines ... Gold often is associated with the presynaptic or postsynaptic cell membranes (arrowheads)" Fig.5: "Distribution of immunogold particles at mossy fiber synapses" - Literal: "Analysis of the gold particles measured within 50 nm from the presynaptic and postsynaptic membrane revealed that KA1 and KA2 are concentrated within 20-30 nm of the synaptic membrane (Fig. 5). For KA1 subunits, the major peak of distribution was on the presynaptic membrane, and a smaller peak of distribution was near the postsynaptic membrane in both synaptic and extrasynaptic zones. Although there is a substantial concentration at synaptic sites, the relative amounts of presynaptic versus postsynaptic labeling is a little more prevalent in extrasynaptic sites. KA2 subunits were located predominantly on the postsynaptic membrane in synaptic and extrasynaptic areas (Fig. 5, graphs)." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Review on MF synaptic transmission where KAR1 and KAR2 are considered to be present in the PSD membrane. @ PMID:16261180 |
| SynGO annotation ID: | 1456 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |