| Annotated protein: | Spectrin beta chain. Gene symbol: SPTBN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q68FG2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SPTBN2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynapse organization (GO:0099173) |
| Synapse type(s): | cerebellum, glutamatergic Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Gao Y, et al. "beta-III spectrin is critical for development of purkinje cell dendritic tree and spine morphogenesis" J Neurosci. 2011 Nov 16;31(46):16581-90 PMID:22090485 |
| Figure(s): | Fig2, fig3 and fig4 |
| Annotation description: | Fig2C and D: Purkinje cells from betaIII spectrin null mice have a lower spine density in culture. This is verified in intact tissue in Fig3, where betaIII spectrin null mice have a lower spine density in Purkinje neurons while the spines of pyramidal neurons are unaltered. This leads to an overall loss of synapses in the cerebellum as shown in fig4, while synapses in the CA1 of hippocampus are spared. However, betaII spectrin seems to fulfill a similar role in cultured hippocampal neurons (see additional ref PMID 28576936) |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Wide-field fluorescence |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | This paper shows that betaII spectrin in essential for spines. Fig5: knockdown of betaIII spectrin results in less spines in cultured hippocampal neurons (but no rescue exp shown), while synapsin staining is normal (Fig6), indicating that synapse formation function of spectrin is postsynaptically driven as is reported in Purkinje cells. @ PMID:28576936 |
| SynGO annotation ID: | 1441 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |