| Annotated protein: | Actin-related protein 2 (Actin-like protein 2). Gene symbol: ACTR2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5M7U6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTR2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | forebrain |
| Annotated paper: | Peng J, et al. "Semiquantitative proteomic analysis of rat forebrain postsynaptic density fractions by mass spectrometry" J Biol Chem. 2004 May 14;279(20):21003-11 PMID:15020595 |
| Figure(s): | Table S1 |
| Annotation description: | Adult rat forebrains were isolated, homogenized and then centrifuged multiple times (including one spin with a sucrose gradient) to yield postsynaptic densities (PSDs). PSDs were then separated on a 1D SDS gel and 14 gel slices were cut from this gel. The slices were trypsinzied and then separated using LC-MS/MS. MS/MS spectra were then compared to the NCBI database using the Sequest algorithm to identify peptides. 374 proteins were found in the PSD. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | none of the above |
| Evidence tracking, Experiment Assay: | Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 1436 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |