Annotated protein: | Neuronal acetylcholine receptor subunit beta-2 (Neuronal acetylcholine receptor non-alpha-1 chain) (N-alpha 1). Gene symbol: CHRNB2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P12390 |
antibody wiki: | |
SynGO gene info: | SynGO data @ CHRNB2 |
Ontology domain: | Cellular Component |
SynGO term: | integral component of presynaptic membrane (GO:0099056) |
Synapse type(s): | striatum |
Annotated paper: | Garcao P, et al. "Subsynaptic localization of nicotinic acetylcholine receptor subunits: a comparative study in the mouse and rat striatum" Neurosci Lett. 2014 Apr 30;566:106-10 PMID:24607281 |
Figure(s): | fig 2A |
Annotation description: | Detection of nAchRs subunits in sub synaptic fractions of rodent striatal synaptosomes with Western Blot 22/11/2017 Pim - The authors mention enrichment of AZ, but I allow annotation to the less specific term presynaptic membrane to reflect the dirty nature of the preparation. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
Evidence tracking, Biological System: | Intact tissue |
Evidence tracking, Protein Targeting: | Antibody (detection) |
Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
SynGO annotation ID: | 1422 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |