| Annotated protein: | Neuronal acetylcholine receptor subunit alpha-4. Gene symbol: CHRNA4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P09483 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHRNA4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | striatum |
| Annotated paper: | Garcao P, et al. "Subsynaptic localization of nicotinic acetylcholine receptor subunits: a comparative study in the mouse and rat striatum" Neurosci Lett. 2014 Apr 30;566:106-10 PMID:24607281 |
| Figure(s): | fig 2A |
| Annotation description: | Detection of nAchRs subunits in sub synaptic fractions of rodent striatal synaptosomes with Western Blot 22/11/2017 Pim - The authors mention enrichment of AZ, but I allow annotation to the less specific term presynaptic membrane to reflect the dirty nature of the preparation. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1420 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |