| Annotated protein: | ADP-ribosylation factor 4. Gene symbol: ARF4. Taxonomy: Mus musculus (Mouse). Uniprot ID: P61750 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARF4 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse organization (GO:0050807) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Jain S, et al. "Arf4 determines dentate gyrus-mediated pattern separation by regulating dendritic spine development" PLoS One. 2012;7(9):e46340 PMID:23050017 |
| Figure(s): | Fig5, Fig8 and Fig9 |
| Annotation description: | Fig5A-C: decrease of spines (esp mushroom type) and 5D-E mini-amplitude in DG-granule cell synapse of ARF4 heterozygous mice. This is selective for the DG, no effects are observed in CA1 (Fig4). Knockdown of ARF4 also results in a reduction of spine number in cultured neurons which can be reverted by re-expressing AFR4 (Fig9) and overexpression of (GTP-locked) ARF4 increses spine number (Fig8). Together these data suggest that ARF4 regulates the number of mature/mushroom spines. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1392 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |