| Annotated protein: | Eps-15 protein (SD09478p). Gene symbol: EPS-15. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: Q8WQ61 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPS15 SynGO data @ EPS15L1 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of presynaptic endocytic zone membrane (GO:0098894) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Koh TW, et al. "Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development" J Cell Biol. 2007 Jul 16;178(2):309-22 PMID:17620409 |
| Figure(s): | fig 2 |
| Annotation description: | In figure 2 the authors use antibodies to demonstrate that Eps15 is enriched in the neuromuscular junction by both confocal and EM. As noted by Pim, the authors point out: 1. "..Collectively, we conclude that Eps15 is widely expressed but is enriched in the neuropil and in the presynaptic compartment of the NMJ." 2. "...reveals that Eps15 is associated with vesicles in the lumen of boutons..." 3. "...Interestingly, a subset of Eps15 is localized to the rim of invaginating vesicles or pits at the plasma membrane in stimulated shibirets1 mutant boutons... |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 1363 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |