| Annotated protein: | Protein phosphatase 3 catalytic subunit alpha (EC 3.1.3.16) (CAM-PRP catalytic subunit) (Calcineurin A alpha) (Calmodulin-dependent calcineurin A subunit alpha isoform) (CNA alpha) (Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform). Gene symbol: PPP3CA. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63328 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP3CA |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic modulation of chemical synaptic transmission (GO:0099170) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Malleret G, et al. "Inducible and reversible enhancement of learning, memory, and long-term potentiation by genetic inhibition of calcineurin" Cell. 2001 Mar 9;104(5):675-86 PMID:11257222 |
| Figure(s): | Fig 2a, b, Fig4. |
| Annotation description: | Authors generated a transgenic mice expressing the auto inhibitory domain in the C terminus of CNAalpha with the rtTA system. These animals were crossed with mice expressing rtTA in neurons under the control of the CaMKIIalpha promoter. Treatment with doxycycline induced the expression of the inhibitory domain of CN and decreased the CN activity in hippocampus and cortex. Induction of LTP in the Schaffer collateral pathway from hippocampal slices prepared from those mice revealed increased LTP compared to control, in agreement with a role of CN works as an antagonist of LTP. This effect was dependent on PKA activity. Because PPF, which is an index of release probability, was facilitated in mutant animals, the effect on LTP is believed to be caused by postsynaptic mechanisms. The enhancement of LTP was also reproduced in vivo using anesthetized and awake mice. In this case, LTP was assessed in the Schaffer collateral pathway but also in dentate gyrus. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Field recordings |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 1346 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |