| Annotated protein: | Cadherin-9 (T1-cadherin). Gene symbol: CDH9. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70407 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CDH9 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Williams ME, et al. "Cadherin-9 regulates synapse-specific differentiation in the developing hippocampus" Neuron. 2011 Aug 25;71(4):640-55 PMID:21867881 |
| Figure(s): | Figure 5 |
| Annotation description: | The work shows CDH-9 immunofluorescence in the proximity of synaptic processes. Transfected expression of a tagged version of the CDH-9 indicates a discrete localization of the recombinantly expressed product outside but close to synaptic cluster. Overall evidence suggest targeted expression. Figure 8 highlights shRNA knock down of the CDH-9 proteins exerts a disruption of synaptic structure supporting synaptic function of the protein as assessed by quantitative morphometric EM. 3/7/2017 Pim - Fig.5C shows the homophilic adhesive property of Cadh9 - This paper can be used to provide CC annotation for Cadh9 based on expression in DG and CA3 Neurons, the co-loc. with PSD-95 and VGlut1, and knowledge of homophilic interactions. Would you feel confident making annotations to "integral component of postsynaptic membrane (GO:0099055)" and "integral component of presynaptic membrane (GO:0099056)" terms? I can assist if you want. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | This paper indicates the specificity controls used for the CDH-9 antibody used in this study. Authors of the annotated paper do hint at a limitation of its usefulness. @ PMID:17668045 |
| SynGO annotation ID: | 1312 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |