| Annotated protein: | Cadherin-9 (T1-cadherin). Gene symbol: CDH9. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70407 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CDH9 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse assembly (GO:0007416) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Williams ME, et al. "Cadherin-9 regulates synapse-specific differentiation in the developing hippocampus" Neuron. 2011 Aug 25;71(4):640-55 PMID:21867881 |
| Figure(s): | Fig.6,7,8 |
| Annotation description: | The work shows CDH-9 immunofluorescence in the proximity of synaptic processes. Transfected expression of a tagged version of the CDH-9 indicates a discrete localization of the recombinantly expressed product outside but close to synaptic cluster. Overall evidence suggest targeted expression. Figure 8 highlights shRNA knock down of the CDH-9 proteins exerts a disruption of synaptic structure supporting synaptic function of the protein as assessed by quantitative morphometric EM. 3/7/2017 Pim - Fig.5C shows the homophilic adhesive property of Cadh9 - Fig.6/7/8 shows that pre-synaptic Cadh9 regulates postsynaptic development and vice versa. In SynGO we have a term synapse adhesion between pre- and post-synapse (GO:0099560) and I think this is very appropriate. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | This paper indicates the specificity controls used for the CDH-9 antibody used in this study. Authors of the annotated paper do hint at a limitation of its usefulness. @ PMID:17668045 |
| SynGO annotation ID: | 1311 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |