| Annotated protein: | Gamma-aminobutyric acid receptor subunit alpha-6 (GABA(A) receptor subunit alpha-6). Gene symbol: GABRA6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P30191 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRA6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | cerebellum, GABAergic |
| Annotated paper: | Nusser Z, et al. "The alpha 6 subunit of the GABAA receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells" J Neurosci. 1996 Jan;16(1):103-14 PMID:8613776 |
| Figure(s): | fig. 3B, D-E, fig. 4A,C |
| Annotation description: | GABA A alpha 6 subunit detection with immuno EM in Golgi cell terminals and mossy fiver terminals. Authors do not explicitly say whether immonereactivity is pre- or postsynaptic. However figures suggest both pre and post. 22/11/2017 Pim - The data presented in this paper shows clear localization to the synaptic specialization of GABA a1 and a6 subunits. However, as the expert noticed, whether this localization related to pre- or postsynaptic localization is not clear. The authors seem to spend an entire section in the discussion on this topic ("Comparison of the pre- and postembedding immunogold localization of receptors"): "However, in the postembedding method the surface of the ultrathin section is reacted and the particles can be displaced from the epitope(s) by -20 nm, which is greater than the width of the membrane. Therefore, the postembedding immunogold method is not reliable to determine the location of the epitope(s) in relation to the two faces of the plasma membrane. " Unfortunately, synapses are always immuno-negative with the pre-embedding method and synaptic observations were only made via postembedding. 23/11/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1300 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |