| Annotated protein: | Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein). Gene symbol: NEFH. Taxonomy: Mus musculus (Mouse). Uniprot ID: P19246 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NEFH |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic intermediate filament cytoskeleton (GO:0099160) |
| Synapse type(s): | hippocampus striatum |
| Annotated paper: | Yuan A, et al. "Neurofilament subunits are integral components of synapses and modulate neurotransmission and behavior in vivo" Mol Psychiatry. 2015 Aug;20(8):986-94 PMID:25869803 |
| Figure(s): | Fig2 and FigS2 |
| Annotation description: | Fig2A-C shows immunoEM localization of NFL (15nm gold, arrowheads) and NFH (10nm gold, arrows) in postsynaptic compartments with little staining in the presynaptic terminal. This is further confirmed by an alternative pre-embedding immuno-EM methods on mouse hippocampus in Supplementary figure 2, where panel F shows immunoreactivity for NFL and panel G for NFH. This reactivity is absent in the TKO lacking NFL, NFH and internexin (quantified in panel I), indicating that the antibody is specific. However, no quantification of pre- and post-synaptic immuno-gold localisation is provided. In the text (p987) the authors state "Similar to NFM labeling, more labeling of NFL, NFH and α-internexin was seen in postsynaptic areas than in presynaptic areas." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1289 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |