| Annotated protein: | Dynamin-2 (EC 3.6.5.5) (Dynamin UDNM). Gene symbol: DNM2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P39054 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DNM2 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | neocortex |
| Annotated paper: | Raimondi A, et al. "Overlapping role of dynamin isoforms in synaptic vesicle endocytosis" Neuron. 2011 Jun 23;70(6):1100-14 PMID:21689597 |
| Figure(s): | fig 2, fig 4 |
| Annotation description: | The authors use dynamin 1 knock out (KO) mice, dynamin 3 KO mice and dynamin 1/3 double KO mice. Using cultured cortical neurons with vGlut1-pHlourin, they demonstrate in fig 4B that fluorescence recovery after stimulation, which is indicative of synaptic vesicle (SV) endocytosis is decreased in dynamin 1 KO mice, is not effected in dynamin 3 KO mice, but is dramatically reduced in dynamin 1/3 double KO mice. Thus, dynamin 1 and 3, which together account for the overwhelming majority of brain dynamin, cooperate in supporting optimal rates of synaptic vesicle endocytosis However, the authors also note that the dynamic 1/3 double KO mice survive for several hours after birth indicates that synaptic transmission must occur in at least a limited form in the absence of both dynamin 1 and 3. Accordingly, electron microscopy analysis of neonatal DKO brain stem synapses (Figures 2C and 2D) revealed the presence of synaptic vesicles and clathrin-coated endocytic intermediates. The supposition is that dynaminm 2 compensates for the loss of dynamic 1 and 3. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Optical physiology |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 1279 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |