Annotated protein: | Ras-related protein Rab-5A (EC 3.6.5.2) (Small GTP-binding protein rab5). Gene symbol: RAB5A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: M0RC99 |
antibody wiki: | |
SynGO gene info: | SynGO data @ RAB5A |
Ontology domain: | Cellular Component |
SynGO term: | anchored component of synaptic vesicle membrane (GO:0098993) |
Synapse type(s): | hippocampus cerebrum |
Annotated paper: | Pavlos NJ, et al. "Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis" J Neurosci. 2010 Oct 6;30(40):13441-53 PMID:20926670 |
Figure(s): | Table 1, Figs 2 and 4 |
Annotation description: | Rab5A was detected by mass spectrometry on highly purified synaptic vesicles. This was further validated using anti-Rab5 antibody by immunoblotting and by staining of GFP-Rab5A with synaptophysin in transiently transfected hippocampal neurons. The presence of Rab5A in synaptic vesicles was separately identified in another study (Takamori et al., 2006; PMID 17110340) by mass spectrometry. |
Evidence tracking, Biological System: | Intact tissue Cultured neurons |
Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Mass-spectrometry Biochemical fractionation (generic) |
Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
Additional literature: | The presence of Rab5A in synaptic vesicles was separately identified in another study (Takamori et al., 2006; PMID 17110340) by mass spectrometry. @ PMID:17110340 |
SynGO annotation ID: | 126 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |