| Annotated protein: | Tomosyn. Gene symbol: TOM-1. Taxonomy: Caenorhabditis elegans (Worm). Uniprot ID: Q49HI2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STXBP5 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of presynaptic dense core vesicle exocytosis (GO:0099161) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Gracheva EO, et al. "Tomosyn negatively regulates CAPS-dependent peptide release at Caenorhabditis elegans synapses" J Neurosci. 2007 Sep 19;27(38):10176-84 PMID:17881523 |
| Figure(s): | Figs. 1-5 |
| Annotation description: | Gracheva et al assess peptide secretion in C. elegans tomosyn-1 mutants and demonstrate a 50% reduction of presynaptic dense core vesicles corresponding to enhanced peptide release. In contrast, overexpression of the protein leads to accumulation of DCVs (Fig. 1). In order to correlate these EM observations directly to enhanced peptide release, the authors apply an imaging assay to measure the amount of secreted YFP-tagged neuropeptide NLP-21 (the neuropeptide is taken up by coelomocytes, whose fluorescent intensity provides an indirect measurement of neuropeptide secretion) and demonstrate increased peptide release in tom-1 mutants. These observation suggests that tom-1 mut effects DCV exocytosis rather than DCV biogenesis. In order to solidify the effect of tom-1 on DCV priming, the authors investigate the functional interplay between tom-1 and DCV priming factor unc-31 (CAPS). Gracheva shows that whereas unc-31 mutation exhibits accumulation of DCV, tom-1 mut partially diminishes the latter, suggesting a mechanisms of tom-1 restriction of unc-31-dependent fusion (fig. 4). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence Optical physiology |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| SynGO annotation ID: | 1259 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |