| Annotated protein: | Syntaxin-binding protein 5 (Lethal(2) giant larvae protein homolog 3) (Tomosyn-1). Gene symbol: STXBP5. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8K400 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STXBP5 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of neuronal dense core vesicle membrane (GO:0098674) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Geerts CJ, et al. "Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains" PLoS One. 2017 Jul 26;12(7):e0180912 PMID:28746398 |
| Figure(s): | Fig 2 H, P; Fig 4, Fig 5 C, F |
| Annotation description: | In this report, Geerts et al study the dynamic distribution of tomosyn-1 in mouse hippocampal neurons. By applying immunoelectron microscopy, immunocytochemistry and live imaging, the authors show localization of tomosyn-1 to dense core vesicles in EM images and indirectly by co-labelling with the DCV marker chromogranin B (Fig 2, 4). Furthermore, time-lapse images and coresponding kymographs supports these findings by showing co-migration tomosyn with neuropeptide Y, a DCV marker (Fig. 5). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Fig. 3B: In the frame of a study on the inhibitory effect of tomosyn on DCV exocytosis, Gracheva et al perfom immunogold labelling of NMJ in C elegans and in line with the primary reference, show localization of tomosyn-1 on dense-core vesicles. @ PMID:17881523 |
| SynGO annotation ID: | 1254 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |