| Annotated protein: | Syntaxin-binding protein 5 (Lethal(2) giant larvae protein homolog 3) (Tomosyn-1). Gene symbol: STXBP5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WU70 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STXBP5 |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic vesicle (GO:0008021) |
| Synapse type(s): | brain |
| Annotated paper: | Takamori S, et al. "Molecular anatomy of a trafficking organelle" Cell. 2006 Nov 17;127(4):831-46 PMID:17110340 |
| Figure(s): | table 1, Suppl Fig S3 |
| Annotation description: | Takamori et al show by subcellualr fractionation of a rat brain followed by proteomic analysis of CPG-purified SVs and immunoblotting of the fractions that tomosyn is enriched in SVs. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Fig. 5A-C, G and Fig. 6: By means of immunoprecipitation of endogeneous neuronal tomosyn and immunostainings in hippocampal neurons, Cazares et al demonstrate the formation of a tomosyn-1, Rab3a-GTP and synapsin 1a/b complex, which is suggested to orchestrate SV partitioning in different SV pools. However, additional experimental evidence for the association of these vesicular peripheral proteins with synaptic vesicles is required since by IPs, no synaptophysin co-enrichment is detected, although colocalization by immunofluorescent co-labelling is observed (Fig. 1 A1-A2). @ PMID:27807164 Geerts et al study the subcellular localization of tomosyn 1 by means of immunofluorescent and immunogold EM staining in neurons. The authors show colocalization with synaptic vesicle markers such as synaptotagmin-1 and VAMP-2 and quantify the fluorescent signal overlap (Fig. 2). Furthermore, they demonstrate association of the protein to SVs and DCV by immunogold labeling (Fig. 2 N-Q). @ PMID:28746398 |
| SynGO annotation ID: | 1250 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |