| Annotated protein: | Syntaxin-1A (Neuron-specific antigen HPC-1). Gene symbol: STX1A. Taxonomy: Mus musculus (Mouse). Uniprot ID: O35526 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX1A |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle exocytosis (GO:0016079) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Vardar G, et al. "Distinct Functions of Syntaxin-1 in Neuronal Maintenance, Synaptic Vesicle Docking, and Fusion in Mouse Neurons" J Neurosci. 2016 Jul 27;36(30):7911-24 PMID:27466336 |
| Figure(s): | Figs 5-7 |
| Annotation description: | This paper studies the function of Syntaxin 1A and Syntaxin 1B using conditional deletion of Stx1B on an Stx1A-null background. To circumvent problems with early lethality in Stx1A/1B double knock-out (DKO) and redundancy in single KO mice. Loss of Stx1 completely abolished fusion-competent vesicles and severely impaired vesicle docking. Partial SNARE complex assembly with the SNARE motif mutant Stx1AAV (A240V, V244A) was sufficient to rescue vesicle docking and neuronal survival but not neurotransmission 24/10/2017 Pim 1. The authors explain that there is functional redundancy between the Stx1A and Stx1B paralogs. Literal: "Investigation of Stx1 function in neurotransmitter release has been rendered impracticable in mammals for two main reasons: mutual compensatory effects of Stx1A and Stx1B (Fujiwara et al., 2006; Gerber et al., 2008; Arancillo et al., 2013; Zhou et al., 2013; Wu et al., 2015)" 2. Authors show that SV fusion is abolished in a cDKO animal. 3. From (1) and (2) it can be inferred that both Stx1A and Stx1B are involved in SV fusion. This is confirmed in part by the complete rescue by over expression of Stx1A in the cDKO animal. - Together, I created annotations for the Stx1A and Stx1B proteins - In section "Stx1 function in vesicle docking and fusion can be uncoupled" the authors show that Stx1A/B functions both docking and fusion. I agree to the less specific term 'synaptic vesicle exocytosis (GO:0016079)' selected by the author. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | PMID:18703708 |
| SynGO annotation ID: | 1247 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |