| Annotated protein: | Glutamate receptor ionotropic, kainate 5 (GluK5) (Glutamate receptor KA-2) (KA2). Gene symbol: GRIK5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63273 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIK5 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Feligioni M, et al. "Ultrastructural localisation and differential agonist-induced regulation of AMPA and kainate receptors present at the presynaptic active zone and postsynaptic density" J Neurochem. 2006 Oct;99(2):549-60 PMID:16903873 |
| Figure(s): | Fig.5-6 |
| Annotation description: | Figure 5. KA2 (Kainate receptor subunit) measured with western blot in pre- and post-synaptic fractions (note that while detected presynaptically, it was enriched in postsynaptic fraction). 8/9/2017 Pim - Experiments involve quantification of plasma membrane/surface levels of receptors via biotinylation assay. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1202 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |