| Annotated protein: | Syntaxin-4. Gene symbol: STX4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q08850 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX4 |
| Ontology domain: | Biological Process |
| SynGO term: | exocytic insertion of neurotransmitter receptor to postsynaptic membrane (GO:0098967) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kennedy MJ, et al. "Syntaxin-4 defines a domain for activity-dependent exocytosis in dendritic spines" Cell. 2010 Apr 30;141(3):524-35 PMID:20434989 |
| Figure(s): | Fig. 5-7 |
| Annotation description: | Kennedy et al aim to provide experimental evidence for exocytosis at dendritic postsynaptic membranes. The authors take advantage of a two-color reporters by fusing transferrin receptor and/or GluA1 to mCherry and SEP-tag (PHluorin variant) in order to monitor trafficking of RE. Furthermore, the authors demonstrate by shRNA and expression of dominant negative soluble version of stx4 that the protein is required for exocytosis of RE. Both approaches reduce transferrin cycling to the PM in response to stimulation (glycine/bicuculline). Kennedy et al aim to validate their findings by a controversial method of whole IgG-mediated cross-linking of exogenously HA-tagged syntaxin-4 prior to stimulation and observe reduced exocytosis (by means of fluorescent detection of RE cargo translocation to the membrane). Although these results only indirectly suggest involvement of Q SNARE molecule syntaxin 4 in postsynaptic RE exocytosis, they remain controversial (see Jurado et al 2013, Neuron and additional literature). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Electrophysiology (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Fig. 6-8: The authors primarily focus on the role of sematodendritic Rab17 isoform on KAR and AMPAR trafficking in hippocampal neurons. Mori et al show that Rab17 colocalizes in the soma, dentritic shafts, tips of developing neurons and in spines with syntaxin 4 and that the interaction is relevant for syntaxin-4's subcellular distribution. Both Rab17 or/and syntaxin-4 KDs affect surface insertion of GluK2 subunit of kainate receptors without influencing GluA1 surface exposure. These findings indirectly suggest that syntaxin 4 is involved in SNARE-mediated fusions postsynaptically. Despite pending controversy regarding the Q SNARE syntaxin-4's function in neurons , its cognate SNARE interaction partners in neurons remain to be identified. @ PMID:24895134 |
| SynGO annotation ID: | 1187 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |