| Annotated protein: | Syntaxin-4 (Renal carcinoma antigen NY-REN-31). Gene symbol: STX4. Taxonomy: Homo sapiens (Human). Uniprot ID: Q12846 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX4 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Soo Hoo L, et al. "The SNARE Protein Syntaxin 3 Confers Specificity for Polarized Axonal Trafficking in Neurons" PLoS One. 2016 Sep 23;11(9):e0163671 PMID:27662481 |
| Figure(s): | Fig. 1, Fig. S3 |
| Annotation description: | In order to determine the subcellular localization of syntaxin 4, the authors of this report clone double myc tag C-terminally to the TMD of syntaxin 4 and thus enable surface expression assessment without cell permeabilization during the staining procedure. The protein show clear punctated surface expression, especially in dendrites. Permeabilization prior to staining with Triton X-100 was used for internal staining protocoll and the results show localization of syntaxin 4 to somatic structures. However, further subcellular localization analysis is not carried out. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | In this report, the authors investigate the role of somatodendritic Rab17 isoform in AMPAR and KAR trafficking. As a part of the experiments, in order to study the subcellular colocalization of rab17 with syntaxin 1-4 isoforms in neurons, Mori et al stain neuronal cells transfected with myc-tagged syntaxin 4 and find that syntaxin 4 is mainly localized to soma, dendritic shafts and spines (Fig. 3-5). @ PMID:24895134 In order to identify proteins involved in postsynaptic exocytosis, Harris et al conduct a screen for proteins disrupting the trafficking of PHluorin-tagged synaptotagmin-4 at Drosophila's NMJ and identify syntaxin 4 as a postsynaptic candidate (fig. 2). @ PMID:27223326 |
| SynGO annotation ID: | 1186 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |