| Annotated protein: | Synaptotagmin-4 (Synaptotagmin IV) (SytIV). Gene symbol: SYT4. Taxonomy: Mus musculus (Mouse). Uniprot ID: P40749 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYT4 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of trans-synaptic signaling by BDNF, modulating synaptic transmission (GO:0150035) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Dean C, et al. "Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release" Nat Neurosci. 2009 Jun;12(6):767-76 PMID:19448629 |
| Figure(s): | Fig.3, 5-8; Fig Suppl 2-5 |
| Annotation description: | Dean et al subcellularly localizes synaptotagmin-4 to brain-derived neurotrophic factor (BDNF)-containing vesicles in hippocampal neurons. By in situ ELISA assay, the authors of the study show that syt-4 negatively regulates BDNF secretion (fig. 3).The authors show that BDNF vesicles undergo exocytosis in axons and dendrites and that syt-4 inhibits the process (Fig. 4 and 5). In addition, by comparison of WT and syt-4 KO animals, Dean et al show that postsynaptic release of BDNF modulate the rate of presynaptic synaptic vesicle exocytosis (retrograde trans-synaptic regulation) (Fig. 6 and 7). Furthermore, the loss of postsynaptic syt-4 increases quantal amplitude and syt-4 animals show enhanced LTP (fig. 8). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Field recordings ELISA |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Yoshihira et al use Drosophila as a model system and show that high frequency stimulation at NMJ can enhance presynaptic miniature release and that this process requires postsynaptic Ca2+ influx and postsynaptic vesicle recycling (utilization of shibire mutant). The authors monitor syt-4 dynamics by using a syt-4 PHluorin expression in postsynaptic muscles. Lack of postsynaptic syt-4 abolishes presynaptic miniature release. Furthermore, syt 4-dependent increase in presynaptic release is shown to be similar to the one observed upon activation of presynaptic cAMP-dependent PKA. Yoshihira et al show that postsynaptic (activity-dependent) modulation of presynaptic morphology and physiology is regulated by presynaptic PKA and postsynaptic syt-4. Based on these findings, a model is suggested, in which postsynaptic retrograde signal is released and regulates trans-synaptically presynaptic cAMP-dependent PKA. @ PMID:16272123 |
| SynGO annotation ID: | 1184 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |