| Annotated protein: | Syntaxin-3. Gene symbol: STX3. Taxonomy: Homo sapiens (Human). Uniprot ID: Q13277 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX3 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Soo Hoo L, et al. "The SNARE Protein Syntaxin 3 Confers Specificity for Polarized Axonal Trafficking in Neurons" PLoS One. 2016 Sep 23;11(9):e0163671 PMID:27662481 |
| Figure(s): | Fig 1 and Fig. 5 |
| Annotation description: | Soo Hoo et al express human Stx3 in rat hippocampal neurons and examine for first time the neuronal cellular distribution of the protein by immunocytochemistry. The authors observe that stx3 is polarized to axons (costaining of axonal marker Tau), with preferential localization at axon tips shortly after adenoviral transduction. Surface expression is assessed by immunostaining using a C-terminal double myc tagged version of the overexpressed protein (tag downstream of the C-terminal transmembrane domain enabling surface detection). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Bennett et al aim to identofy further members of the syntaxin family. cDNA clones from rat cDNA library are sequenced and Northern blot analysis shows broad expression of syntaxin 3 (Fig. 4). @ PMID:7690687 The authors sequence cDNA clones from mouse cDNA library and identify four different isoforms of syntaxin 3 - 3 A, B , C and D, produced from the same gene by alternative splicing. Syntaxin 3D gives rise to two isoforms differing by a single amino acid (87th aa) suggesting single nucleotide polymorphism. @ PMID:7598732 Takamori et al fail to detect syntaxin 3 by mass spectrometry in CPG-purified SVs (Table 1), Moreover, immunoblotting for stx3 of all subcellular fractions does not show enrichment of the protein in SVs as compared to other fractions. This might be an indirect indication for plasma membrane localization. @ PMID:17110340 The authors show by immunostaining of exogeneously expressed double myc tagged human stx3 that in epithelial cells MDCK and nonepithelial cells stx3 forms clusters at the apical plasma membrane. @ PMID:16339081 Fig. 3A, Fig 4 and Fig. 5A: Western blot analysis of rat internal organs and different brain regions confirms ubiquitous expression of syntaxin 3 on protein level (in contrast to missing signal for stx 3 mRNA in Northern blots as described by Bennett et al, Cell 1993, see above). Immunostaining of PC12 cells for endogeneous syntaxin 3 confirms plasma membrane localization of the protein. This data is confirmed also by PMID 16598260. @ PMID:15975093 Syntaxin 3A and 3B isoforms are detected in olfactory bulb, hippocampus and regions of the cortex by in situ hybridization after induction of LTP in vivo. @ PMID:9681457 Morgans et al show for first time that syntaxin 3 is preferentially expressed in ribbon synapses (immunostaining of sections from rat retina - strong syntaxin 3- positive signals detected in OPL and weaker in IPL). Others show later that syntaxin 3B isoform is the one specific for ribbon synapses (see PMID 18683220) @ PMID:8824312 Fig. 1, 2A, Movie S1: Jurado et al assess the postsynaptic role of syntaxins in AMPAR trafficking during LTP and find stx-3 positive spots in immunocytochemistry in dendrites (Fig. 2A and Movie S1). The authors do not show direct colocalization of stx-3 in the ICC images, but in the context of the paper they provide further functional evidence for postsynaptic localization of the protein in dendrites. @ PMID:23395379 |
| SynGO annotation ID: | 1181 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |