| Annotated protein: | Intersectin-1 (EH domain and SH3 domain regulator of endocytosis 1). Gene symbol: ITSN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WVE9-1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ITSN1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic actin cytoskeleton (GO:1905274) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Thomas S, et al. "Intersectin regulates dendritic spine development and somatodendritic endocytosis but not synaptic vesicle recycling in hippocampal neurons" J Biol Chem. 2009 May 1;284(18):12410-9 PMID:19258322 |
| Figure(s): | fig 7, fig 8 |
| Annotation description: | In fig 7, the authors show that intersection 1-long isoform co-localizaes with actin in the post-synapse. In fig 7 they demonstrate that knockdown of intersection 1-long using lentivirus disrupts the development of dendritic spines. 15/8/2017 Pim - It is important to note that: "Intersectin-l is a GEF for Cdc42. In vitro, intersectin-l stimulates the formation of branched actin filaments by activating Cdc42 with subsequent downstream activation of N-WASP (23)." While the paper shows only an effect on spine maturation, the inference is that this is via 'regulation of modification of postsynaptic actin cytoskeleton (GO:1905274)' |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| Additional literature: | Intersectin-l is a GEF for Cdc42. In vitro, intersectin-l stimulates the formation of branched actin filaments by activating Cdc42 with subsequent downstream activation of N-WASP. @ PMID:11584276 |
| SynGO annotation ID: | 1179 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |