Annotated protein:Intersectin-1 (EH and SH3 domains protein 1). Gene symbol: ITSN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z0R4
antibody wiki:
SynGO gene info:SynGO data @ ITSN1
Ontology domain:Biological Process
SynGO term:synaptic vesicle endocytosis (GO:0048488)
Synapse type(s):cerebral cortex
Annotated paper:Yu Y, et al. "Mice deficient for the chromosome 21 ortholog Itsn1 exhibit vesicle-trafficking abnormalities" Hum Mol Genet. 2008 Nov 1;17(21):3281-90 PMID:18676989
Figure(s):fig 5
Annotation description:In figure 5 the authors use cortical neurone generated from their intersection null mice. The neurone are transfected with synaptophlourin (SynpHlourin) and stimulated electrophysiologically to cause exocytosis of the SVs and enhanced SynpHlourin fluorescence. With termination of the stimulus, SV are reformed by endocytosis, causing quenching of the pHlourin signal and this is delayed in KO cultures.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:Optical physiology
Annotator(s):Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
Additional literature:The authors inject an antibody (function blocking) into the pre synapse of reticulospinal neurons in lamprey. They stimulate the neurone for 20 min at 5 Hz causing exo/endocytic recycling of SVs. In the antibody injected neurons, the SV pool is depleted. @ PMID:20160082
SynGO annotation ID:1178
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology