| Annotated protein: | Ras-related protein Rab-5A (EC 3.6.5.2) (Small GTP-binding protein rab5). Gene symbol: RAB5A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: M0RC99 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAB5A |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hoopmann P, et al. "Endosomal sorting of readily releasable synaptic vesicles" Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):19055-60 PMID:20956291 |
| Figure(s): | Fig 2 |
| Annotation description: | Colocalisation of recycling SVs labelled by synaptotagmin and Rab5(Q79L) in neurons indicates that SV endocytosis goes through a Rab5-containing endosomal compartment. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
| Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
| Additional literature: | Figs 2, 5, 6, 7 and 8 Rab5 labels endosomal vesicles in Drosophila presynaptic terminals. In Rab5 mutants, these structures are largely missing in CNS and NMJ presynapses. In addition, dominant-negative Rab5 mutant (S43N) localise diffusely in the presynaptic cytosol, concomitant with the accumulation of 70 nm vesicles. These vesicles are thought to be endocytic intermediates that were unable to fuse with early endosomes. The observation is is partially supported by FM1-43 labelling experiments where a significant decrease in labelling of the recycling SV pool was observed in Rab5(S43N) expressing neurons after the initial loading step, suggesting a defect in the endocytic recycling step. @ PMID:12743108 Role for Rab5A in SV recycling @ PMID:16141272 |
| SynGO annotation ID: | 1169 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |