| Annotated protein: | Syntaxin-1A (Neuron-specific antigen HPC-1) (Synaptotagmin-associated 35 kDa protein) (P35A). Gene symbol: STX1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P32851 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | cerebellum |
| Annotated paper: | Koh S, et al. "Immunoelectron microscopic localization of the HPC-1 antigen in rat cerebellum" J Neurocytol. 1993 Nov;22(11):995-1005 PMID:8301329 |
| Figure(s): | Fig. 3; Fig. 11 |
| Annotation description: | By application of cryoimmunogold labelling on rat cerebellum sections, Koh et al demonstrate that HPC-1 is localized on plasma membrane and synaptic vesicles of presynaptic boutons which form synapses with Purkenje cells (Fig. 3 and Fig. 11). 2/9/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Microscopy (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Miya et al demonstrate the localization of syntaxin -1 to presynaptic nerve terminal plasma membrane and SVs (fig. 4) by immunoelectron microscopy. @ PMID:9078410 The authors demonstrate that major pool of syntaxin 1 recycle with synaptic vesicles (SVs). However, immunoisolation of SVs with synaptophysin and syntaxin 1 specific antibodies show that most of the SVs contain syntaxin 1, but also that syntaxin 1 beads could isolate also the most of Na/K ATPase-positive membrane fragments from the initial material (Fig. 4). This data suggests indirectly that syntaxin 1 is also localized to nerve terminal plasma membrane. @ PMID:7860636 Bennett shows biochemically that syntaxin-1 is membrane bound protein from presynaptic release sites. @ PMID:1321498 |
| SynGO annotation ID: | 1163 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |