| Annotated protein: | Regulating synaptic membrane exocytosis protein 2 (Rab-3-interacting molecule 2) (RIM 2). Gene symbol: RIMS2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JIS1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMS2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone cytoplasmic component (GO:0098831) |
| Synapse type(s): | spinal cord Photoreceptor ribbon synapse |
| Annotated paper: | Wang Y, et al. "Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion" Nature. 1997 Aug 7;388(6642):593-8 PMID:9252191 |
| Figure(s): | Figure 3A, 3C |
| Annotation description: | It is demonstrated with immuno-EM and immunofluorescence that RIM is specifically localizaed at the active zone in spinal cord and ribbon synapses. It makes use of two separate antibodies, plus additional controls (including secondary only). Wang et al. 2016 provides a knockout-control for the immunofluorescence, while Tang et.al. 2016 demonstrates the active zone localization using STORM microscopy. 10/2/2017 Pim: -Fig.3A: spinal cord --> wide-field microscopy -Fig3B: retinal ribbon synapse --> immuno-EM |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Wang et al. 2016 provides a knockout-control for the immunofluorescence @ PMID:27537483 Tang et.al. 2016 demonstrates the active zone localization using STORM microscopy. @ PMID:27462810 |
| SynGO annotation ID: | 116 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |