| Annotated protein: | Synapsin-2 (Synapsin II). Gene symbol: SYN2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63537 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYN2 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of synaptic vesicle membrane (GO:0098850) |
| Synapse type(s): | neocortex |
| Annotated paper: | Thiel G, et al. "Synapsin II. Mapping of a domain in the NH2-terminal region which binds to small synaptic vesicles" J Biol Chem. 1990 Sep 25;265(27):16527-33 PMID:2118908 |
| Figure(s): | Fig. 5 |
| Annotation description: | Thiel et al. isolated synaptic vesicle from rat brain to investigate how synapsin II is anchored to the vesicle membrane. To this end, they used salt extraction and limited proteolysis by trypsin, and they assessed binding of recombinant synapsin II to synaptic vesicles. Synapsin II was partially degraded by limited proteolysis. Moreover, part of the protein was removed by high ionic strength buffers suggesting that the protein is a peripheral membrane protein of synaptic vesicles. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Immunocytochemistry experiments on the culture of neurons revealed that synapsin 2 has a high colocalization with synaptophysin, a synaptic vesicles marker, (Fig 4F). @ PMID:10804215 |
| SynGO annotation ID: | 1159 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |