| Annotated protein: | Adenosine receptor A3 (A3AR). Gene symbol: ADORA3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61618 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADORA3 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Garcia N, et al. "Adenosine A2B and A3 receptor location at the mouse neuromuscular junction" J Anat. 2014 Jul;225(1):109-17 PMID:24754634 |
| Figure(s): | Fig. 1 and 3 |
| Annotation description: | Immunolabellings of neuromuscular junctions using two different commercial antibodies directed against adenosine A3 receptors were performed. These experiments indicated the presence of A3Rs at NMJ synapses. A3R immunoreactivity did not co-localize with the glial marker S-100, but with the presynaptic membrane marker syntaxin and the muscle membrane marker acetylcholine receptors. The specificity of the antibodies was assessed by Western blot and pre-incubation of the primary antibody with a control peptide (see material and methods). Further, primary antibody was omitted for IHC to control for unspecific staining by the secondary antibodies. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1153 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |