| Annotated protein: | Synaptotagmin-4 (Synaptotagmin IV) (SytIV). Gene symbol: SYT4. Taxonomy: Mus musculus (Mouse). Uniprot ID: P40749 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYT4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of neuronal dense core vesicle membrane (GO:0099066) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Dean C, et al. "Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release" Nat Neurosci. 2009 Jun;12(6):767-76 PMID:19448629 |
| Figure(s): | Fig. Suppl 1 and Fig. 1 |
| Annotation description: | In order to assess the subcellular localization of synaptotagmin 4, Dean et al perform series of immunocytochemical stainings on cultured hippocampal neurons from WT and syt4 KO mice ( Suppl Fig 1 and Fig. 1). Co-staining for endogeneous syt-4 in BDNF-GFP (brain-derived neurotrophic factor) transfected hippocampal neurons reveals colocalization of both proteins in axons and dendrites. In addition, the authors constructed an mCherry-tagged fusion syt-4 that colocalizes with BDNF-GFP, exhibiting a pattern similar to that of endogeneous synaptotagmin-4 (the specificity of the syt-4 antibody is verified by using neurons from syt-4 KO mice). Moreover, both proteins are shown to move together in an activity-dependent manner. Thus, it can be concluded that syt-4 is colocalized to BDNF-positive vesicles in hippocampal neurons. Furthermore, the immunostaining data shows prominent staining for syt 4 in Golgi (co-staining for GM130 as a marker protein, Suppl S1). This observation is in line with findings of others (see additional literature) |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Fig. 2, Fig 5: The paper reports localization of syt 4 to Golgi in hippocampal neurons and differentiated PC12 cells. @ PMID:10646502 Fig. 6: Localization of syt 4 to Golgi in cultured hippocampal neurons @ PMID:9006966 |
| SynGO annotation ID: | 1148 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |