| Annotated protein: | Synaptotagmin-6 (Synaptotagmin VI) (SytVI). Gene symbol: SYT6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62746 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYT6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic membrane (GO:0099699) |
| Synapse type(s): | brain |
| Annotated paper: | Butz S, et al. "The subcellular localizations of atypical synaptotagmins III and VI. Synaptotagmin III is enriched in synapses and synaptic plasma membranes but not in synaptic vesicles" J Biol Chem. 1999 Jun 25;274(26):18290-6 PMID:10373432 |
| Figure(s): | Fig. 6-8 |
| Annotation description: | Butz et al report in this paper that synaptotagmin VI is localized at synaptic plasma membranes. The authors generate a specific antibody and verify it by using brain material from a synaptotagmin VI KO mouse (unpublished). Immunoblotting of fractions derived from rat brain subcellular fractionation experiments shows no enrichment of synaptogmin VI (though remaining detectable) in synaptic vesicle fractions. In contrast, the protein is evenly detected in all subcellular fractions (Fig. 6). Additionally, in order to test whether synaptotagmin VI is a SV protein, the authors immunoblot ([125]I-secondary-antibody-detection) synaptosomes, crude SV fraction and CPG-isolated SVs for a set of vesicular proteins and observe decrease in synaptotagmin VI signal compared to that of the other SV marker proteins, e.g. synaptogyrin I, which is used for normalization (Fig. 7). Synaptic vesicle immunoisolation via anti-synaptotagmin-1 or anti-synaptobrevin 2 antibodies and immunodetection (as mentioned above) reveals no signal for synaptotagmin VI in the synaptic vesicles. This finding is consistent with the absence of synaptotagmin VI on SV as reported by Takamori, S et al (Cell, 2006) 6/2/2018 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Radioimmunoassay |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Fukuda and Mikoshiba report alternatively spliced forms of synaptotagmin VI in mouse brain - one with transmembrane domain and one soluble plasma membrane-associated lacking 85 N-terminal aa including the TMD (Fig. 1, 4, 5 and 8). However, further investigation is required in order to address the subcellular localization as well as the function of the isoforms in CNS @ PMID:10531344 |
| SynGO annotation ID: | 1145 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |