| Annotated protein: | Syntaxin-binding protein 5 (Lethal(2) giant larvae protein homolog 3) (Tomosyn-1). Gene symbol: STXBP5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WU70 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STXBP5 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of presynaptic membrane (GO:0098888) |
| Synapse type(s): | cerebellum |
| Annotated paper: | Fujita Y, et al. "Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process" Neuron. 1998 May;20(5):905-15 PMID:9620695 |
| Figure(s): | Fig 6 |
| Annotation description: | In order to determine the subcellular localization of tomosyn-1, Fujita and colleagues divide rat cerebra in three fractions: cytosolic, detergent-soluble and detergent-insoluble fraction and perform immunoblotting for tomosyn-1 (after 1% Triton X-100 solubilization followed by 1h centrifugation at 100 000 x g). The protein is detected predominantly in the first two fractions (Fig. 6Ba). Furthermore, the authors fractionate rat synaptosomal fraction into cytosol, synaptic plasma membrane and synaptic vesicle fraction and detect highest enrichment of tomosyn-1 in the synaptic membrane fraction and also to some extent in the cytosolic fraction (Fig 6Bb). These results are in line with the observation of immunogold labelling of rat cerebellar molecular layer (Fig 6Da,c) and neuromuscular junction (Fig. 6Db,d). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Groffen et al investigate the brain distribution of seven tomosyn isoforms derived from two distinct genes by in situ hybridization and qRT-PCR. The findings suggest strong increase in tomosyn-2 expression during development. @ PMID:15659226 The authors investigate the interaction between syntaxin 1A and tomosyn in bovine adrenal chromaffin cells. Gladycheva et al show that exogenously expressed CFP-tomosyn and cYFP-syntaxin 1A are localized to the plasma membrane region. In addition, by using both acceptor photobleach and sensitized emission FRET the authors show that the localization of tomosyn to the plasma membrane is due to direct bimolecular interaction with syntaxin 1A (fig. 3A and D). Furthermore, by mutational analysis it is shown that the tomosyn-syntaxin 1A FRET signal is due to an interaction between the SNARE motifs of syntaxin and tomosyn (fig. 3 B and F). Moreover, the authors show that re-localization of endogeneous tomosyn from the cytoplasm to the membrane is increased by stimulation (fig. 4 and 5, as assessed by immunoprecipitation of tomosyn under NSF blocking conditions and agonist activation and in a photobleachign expriment, respectively). @ PMID:17545156 By applying confocal and two-photon high-resolution microscopy, Barak et al demonstrate predominant localization of tomosyn-1 to glutamatergic synapses in mouse hippocampus (high concentration in hilus and mossy fibers; Fig. 4A-C and Fig. 5) @ PMID:21191478 |
| SynGO annotation ID: | 1141 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |