| Annotated protein: | Syntaxin-binding protein 5 (Lethal(2) giant larvae protein homolog 3) (Tomosyn-1). Gene symbol: STXBP5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WU70 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STXBP5 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | cerebellum Neuro-muscular junction Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Fujita Y, et al. "Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process" Neuron. 1998 May;20(5):905-15 PMID:9620695 |
| Figure(s): | Fig. 6 |
| Annotation description: | In order to determine the subcellular localization of tomosyn-1, Fujita and colleagues divide rat cerebra in three fractions: cytosolic, detergent-soluble and detergent-insoluble fraction and perform immunoblotting for tomosyn-1 (after 1% Triton X-100 solubilization followed by 1h centrifugation at 100 000 x g). The protein is detected predominantly in the first two fractions (Fig. 6Ba). Furthermore, the authors fractionate rat synaptosomal fraction into cytosol, synaptic plasma membrane and synaptic vesicle fraction and detect highest enrichment of tomosyn-1 in the synaptic membrane fraction and also to some extent in the cytosolic fraction (Fig 6Bb). These results are in line with the observation of immunogold labelling of rat cerebellar molecular layer (Fig 6Da,c) and neuromuscular junction (Fig. 6Db,d). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Geerts et al perform immunostaining and EM combined with immunogold labelling in order to study the distribution of tomosyn 1 and 2 in neurons. The authors show not only punctated, but rather intense diffuse immunoreactivity for the proteins in dendrites and axons, confirming the (presynaptic) cytosolic localization of the tomosyn (Fig. 1). @ PMID:28746398 |
| SynGO annotation ID: | 1138 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |