| Annotated protein: | Synaptophysin (Major synaptic vesicle protein p38). Gene symbol: SYP. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P07825 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYP |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic vesicle membrane (GO:0030285) |
| Synapse type(s): | brain spinal cord retina |
| Annotated paper: | Jahn R, et al. "A 38,000-dalton membrane protein (p38) present in synaptic vesicles" Proc Natl Acad Sci U S A. 1985 Jun;82(12):4137-41 PMID:3923488 |
| Figure(s): | Fig. 1-6; Table 1-2 |
| Annotation description: | In this reference paper, Jahn et al. investigate the distribution of synaptophysin by subcellular fractionation, immunoblotting and immunostaining of coronal sections of rat brain. Additionally, solubilization studies (with Triton X-114 phase separation) and controlled proteolysis of intact and solubilized synaptic vesicles reveal that synaptophysin is an integral vesicular protein. 2/9/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue Cell-free system |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Western blot Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | The data confirms the findings from the primary ref paper: by means of subcellular fractionation combined with immunoblotting and mass spectrometry, Takamori et al show that synaptophysin is the most abundant SV protein and that it's highly enriched in CPG-isolated SVs (Fig. 1B, Table 1 and Fig Suppl. 3) @ PMID:17110340 Fig. 9a-b shows images from immunoelectron microscopy of sections from murine cerebrum and rat synaptosomes: the labelling shows specific binding of anti-synaptophysin I antibody to presynaptic vesicles. @ PMID:3924408 |
| SynGO annotation ID: | 1136 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |