| Annotated protein: | Huntingtin-associated protein 1 (HAP-1). Gene symbol: HAP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P54256 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HAP1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Kittler JT, et al. "Huntingtin-associated protein 1 regulates inhibitory synaptic transmission by modulating gamma-aminobutyric acid type A receptor membrane trafficking" Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12736-41 PMID:15310851 |
| Figure(s): | Fig 2, Fig 3a,b, Fig 4a,b,c, Fig 5 |
| Annotation description: | Authors shows that HAP1 directly interacts with GABAA receptors and can modulate the their fate. HAP1a overexpression in cortical cultures leads to a reduction of the degradation of internalized receptors and induces an increase in the recycling of these. Supporting this, amplitudes but not frequencies of mIPSPs were significantly increased upon HAP1a overexpression which suggests a postsynaptic phenotype. Analysis of the inhibitory synaptic currents revealed that this increase in mIPSP amplitude is due to a increase number of channels in the cell surface. In line with this, cell surface biotynilation revealed an increased surface expression of the beta3 subunit of the channel. Beta subunits are essential for the functional expression of these receptors. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Western blot IP + WB/MSMS |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 1097 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |