| Annotated protein: | Alpha-2A adrenergic receptor (Alpha-2A adrenoreceptor) (Alpha-2A adrenoceptor) (Alpha-2AAR) (Alpha-2D adrenergic receptor) (CA2-47). Gene symbol: ADRA2A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P22909 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADRA2A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic |
| Annotated paper: | Milner TA, et al. "Hippocampal alpha2a-adrenergic receptors are located predominantly presynaptically but are also found postsynaptically and in selective astrocytes" J Comp Neurol. 1998 Jun 8;395(3):310-27 PMID:9596526 |
| Figure(s): | Table 1, Fig. 1, 5, 8, 9 |
| Annotation description: | Pre-embedding immuno-peroxidase labelling for electron microscopy indicates the presence of Alpha2A Adrenergic receptor (Alpha2A-AR) immunoreactivity in a large percentage of axons and axon terminals in the hippocampus (Fig. 5, 8, 9). The majority of the labelled synapses were asymmetric (Fig. 5B, E), but presynaptic immunoreactivity was also found in some symmetric terminals that were contacting somata or dendritic shafts (for example Fig. 5A). Alpha2A-AR immunoreactivity was found near the presynaptic membrane (Fig. 5A,D), but the peroxidase reaction product was also present throughout the presynaptic terminal associated with synaptic vesicles. Preabsorption of the antibody with the peptide completely abolishes immunostaining (Fig. 1C). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1083 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |