| Annotated protein: | Calcium-binding protein 1 (CaBP1) (Caldendrin). Gene symbol: CABP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O88751 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CABP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Laube G, et al. "The neuron-specific Ca2+-binding protein caldendrin: gene structure, splice isoforms, and expression in the rat central nervous system" Mol Cell Neurosci. 2002 Mar;19(3):459-75 PMID:11906216 |
| Figure(s): | Fig. 7 |
| Annotation description: | Fig.7: Immunoperoxidase electron microscopical localisation of Caldendrin in rat cerebral cortex. In the parietal cortex (A-C) cytoplasmic labeling was frequently observed in large to small dendritic pro files (d in A) throughout the cortical layers (see light microscopical results). In synaptic areas, caldendrin immunoreactivity in different cortical layers was exclusively localised postsynaptically (see arrows) in asymmetrical glutamatergic synapses. Labeled synapses (arrows) were either large, complex synapses (A and B), or smaller spine synapses (C). Note that immunopositive synapses are found in close neighbourhood together with unlabeled synapses of the same morphological type (marked by asterisks). (D) In immunopositive putative interneuron dendrites (receiving several asymmetrical synapses) the DAB reaction product displayed - in contrast to all other examples shown-no tendency of being distributed toward the postsynaptic densities asterisks). (E) Occasionally, two synapses on the same target structure and in close neighbourhood were observed to be immunopositive and immunonegative, respectively, for caldendrin. The survey micrograph (G) of caldendrin-IR in the cortex shows that in close neighbourhood to the relatively rarely occurring immunopositive synapses (arrow) many immunonegative synapses (asterisks) can be found. The appearance (staining) of postsynaptic densities in immunonegative synapses (A, B, D, E, G) was similar when comparing them to material obtained from negative controls, which were not incubated with the primary antibody (F). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | PMID:9694893 PMID:1830394 PMID:23296610 |
| SynGO annotation ID: | 108 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |