| Annotated protein: | Sodium- and chloride-dependent glycine transporter 1 (GlyT-1) (GlyT1) (Solute carrier family 6 member 9). Gene symbol: SLC6A9. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P28572 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A9 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | neocortex Mossy fiber synapse (DG->CA3) Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Cubelos B, et al. "Localization of the GLYT1 glycine transporter at glutamatergic synapses in the rat brain" Cereb Cortex. 2005 Apr;15(4):448-59 PMID:15749988 |
| Figure(s): | Figure 6 |
| Annotation description: | Immunohistochemical analysis of GlyT1 expression in neurons. Figure 6 A-F: Pre-embedding immunogold staining of GLYT1 in the hippocampus the neocortex, and the spinal cord. Figure 6 G-I, K: Post-embedding immunogold of GLYT1 in the dentate gyrus and the molecular layer of the cerebellum. The profiles show localization in both the pre- and postsynaptic compartments, as well as in glial processes. Figure 6G shows post-embedding immunogold staining for GLYT1 at the post synaptic density. Figure 6J shows double post-embedding immunogold labeling for GLYT1 and NMDAR in the hippocampus, and demonstrates colocalization at the postsynaptic density. 2/7/2017 Pim - Annotation was not made agains the membrane part of the PSD because staining does not suggest membrane localization. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1073 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |