| Annotated protein: | Alpha-1A adrenergic receptor (Alpha-1A adrenoreceptor) (Alpha-1A adrenoceptor) (Alpha-1C adrenergic receptor). Gene symbol: ADRA1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P43140 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADRA1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | striatum, glutamatergic striatum, GABAergic striatum, dopaminergic |
| Annotated paper: | Mitrano DA, et al. "alpha-1 Adrenergic receptors are localized on presynaptic elements in the nucleus accumbens and regulate mesolimbic dopamine transmission" Neuropsychopharmacology. 2012 Aug;37(9):2161-72 PMID:22588352 |
| Figure(s): | Fig.3, 4 |
| Annotation description: | Pre-embedding immunogold electron microscopy was performed using a commercial antibody directed against Alpha1 adrenergic receptors (aa339-349, rb, polyclonal). Pre-absorption of the antibody with the A1 peptide eliminated the immunolabelling (data not shown, but mentioned in the text). A1 adrenergic receptors (A1ARs) were localized primarily pre-synaptically, but also in dendrites and spines (Fig. 3a) in the nucleus accumbens (NAc), ventral tegmental area (VTA) and the prefrontal cortex (PFC). The majority of the A1ARs were found extrasynaptically (not associated with the postsynaptic specialization or the peri-synaptic sites; Fig. 3c). Co-labelling with different markers revealed that A1ARs are localized to different types of neurons (TH - dopaminergic, vGluT1/2 - glutamatergic, GABA - Gabaergic; Fig. 4). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1071 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |