| Annotated protein: | Alpha-1A adrenergic receptor (Alpha-1A adrenoreceptor) (Alpha-1A adrenoceptor) (Alpha-1C adrenergic receptor). Gene symbol: ADRA1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P43140 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADRA1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | striatum, glutamatergic striatum, GABAergic striatum, dopaminergic |
| Annotated paper: | Mitrano DA, et al. "alpha-1 Adrenergic receptors are localized on presynaptic elements in the nucleus accumbens and regulate mesolimbic dopamine transmission" Neuropsychopharmacology. 2012 Aug;37(9):2161-72 PMID:22588352 |
| Figure(s): | Fig.3, 4 |
| Annotation description: | Pre-embedding immunogold electron microscopy was performed using a commercial antibody directed against Alpha1 adrenergic receptors (aa339-349, rb, polyclonal). Pre-absorption of the antibody with the A1 peptide eliminated the immunolabelling (data not shown, but mentioned in the text). A1 adrenergic receptors (A1ARs) were localized primarily pre-synaptically in unmyelinated axons and axon terminals (Fig. 3a) in the nucleus accumbens (NAc), ventral tegmental area (VTA) and the prefrontal cortex (PFC). ~60% of axonal A1ARs were associated with the plasma membrane (Fig. 3b). The majority of the axonal A1ARs were found extrasynaptically (not associated with the active zone or the peri-active zone; Fig. 3c). Co-labelling with different markers revealed that A1ARs are localized to different types of neurons (TH - dopaminergic, vGluT1/2 - glutamatergic, GABA - Gabaergic; Fig. 4). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1070 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |