| Annotated protein: | Catenin delta-1 (Cadherin-associated Src substrate) (CAS) (p120 catenin) (p120(ctn)) (p120(cas)). Gene symbol: CTNND1. Taxonomy: Homo sapiens (Human). Uniprot ID: O60716 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CTNND1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Ochiishi T, et al. "Regulation of AMPA receptor trafficking by delta-catenin" Mol Cell Neurosci. 2008 Dec;39(4):499-507 PMID:18602475 |
| Figure(s): | Figure 1D, 2,3 |
| Annotation description: | The paper studies the effect of the over expression of delta-Catenin on AMPA receptor surface expression. Figure 1D: A1-A4 show the effect of over expression of the WT delta-catenin in organotipic slices: the AMPA to NMDA ratio increases dramatically, possibly indicating an increase in the fraction of AMPA receptors at the membrane. B1-B4: when a mutant delta-Catenin is expressed where the c-terminal PDZ domain is deleted, the effect is not seen. Figure 2 shows that when data-catenin, GRIP and GluR2 are expressed in COS7 cells, the fraction of membrane GluR2 is higher (as assessed by a biotinilation assay, lanes labelled 'B'). Figure 3 uses immunoflorescence to show that the overexpression of delta-catenin in cultured hippocampal neurons increases the expression of GluR2 (Figure 3A), and GRIP (Fig. 3B) in spines, but not of NMDA receptors (Figure 3C). Taken together the data suggests that delta-catenin over-expression may increase the surface fraction of GluR2 via its interaction with GRIP. The electrophysiology data would argue that at lease a fraction of the inserted AMPA receptors are synaptic. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Electrophysiology (generic) Western blot |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1067 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |