| Annotated protein: | Cell division control protein 42 homolog (EC 3.6.5.2). Gene symbol: CDC42. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8CFN2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CDC42 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic structure (GO:0099159) |
| Synapse type(s): | hippocampus Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Murakoshi H, et al. "Local, persistent activation of Rho GTPases during plasticity of single dendritic spines" Nature. 2011 Apr 7;472(7341):100-4 PMID:21423166 |
| Figure(s): | Fig3 |
| Annotation description: | This paper addresses the role of Rho GTPases in spine morphology changes upon stimulation. The stimulation is provided by glutamate uncaging and the assay is validated in Fig 1. Fig 3 addresses how GTPases RhoA and CDC42 are involved in the postsynaptic volume change upon glutamate uncaging. The data shows that CDC42 knockdown affects the sustained response selectively, and this can can be rescued by expression of shRNA resistant CDC42 (Fig 3E, F, I and J). Similar results are obtain by the acute expression of the CDC42-binding domain of WASP (Fig3G) that functions as a CDC42 inhibitor. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Two-photon microscopy |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | This paper confirms the findings of CDC42 knockdown on spine volume changes in rat slice now in inducible CDC42 knockout mouse neurons. Fig3A & B show that the spines from the CDC42 null mice do not change volume (especially in the sustained phase) upon glutamate uncaging and this can be restored by expression of CDC42 (Fig3C & D). Note tht the transient spine volume changes are not fully restored in Fig 3C, in line with the specific effect of CDC42 on sustained spine morphology after intense stimulation. In parallel, the inducible CDC42 null neurons show impairment in LTP (Fig 3E) which could be the result of the defect in spine morphology changes. @ PMID:25006034 |
| SynGO annotation ID: | 1064 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |