| Annotated protein: | Protein phosphatase 3 catalytic subunit alpha (EC 3.1.3.16) (CAM-PRP catalytic subunit) (Calcineurin A alpha) (Calmodulin-dependent calcineurin A subunit alpha isoform) (CNA alpha) (Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform). Gene symbol: PPP3CA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63329 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP3CA |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | brain |
| Annotated paper: | Kuno T, et al. "Distinct cellular expression of calcineurin A alpha and A beta in rat brain" J Neurochem. 1992 May;58(5):1643-51 PMID:1313851 |
| Figure(s): | Table 2 |
| Annotation description: | In this study authors generated antisera against calcineurin A alpha by immunizing a rabbit and used it to detect calcineurin A alpha in different subcellular fractions prepared from rat brain. Though data showing the relative amounts of calcineurin A alpha in the different fractions is only presented in a table format and blots are not shown, authors claim that Calcineurin A alpha is enriched in the P2C fraction containing synaptosomes, and when this fraction was lysed, 80% of the protein was recovered in the soluble fraction, which suggest that calcineurin A alpha is most abundant in the synaptic cytosol. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| Additional literature: | In this paper authors describe the isolation from a cDNA rat brain library and the sequence of the rat brain calcineurin A alpha. @ PMID:2551293 |
| SynGO annotation ID: | 1062 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |