| Annotated protein: | IQ motif and SEC7 domain-containing protein 1. Gene symbol: IQSEC1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R0S2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IQSEC1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Scholz R, et al. "AMPA receptor signaling through BRAG2 and Arf6 critical for long-term synaptic depression" Neuron. 2010 Jun 10;66(5):768-80 PMID:20547133 |
| Figure(s): | Fig. 1, 2, 6, 5, 7, 8, S3, S4 |
| Annotation description: | GEP100/BRAG2/IQSEC1 is a guanine nucleotide exchange factor for Arf6 at excitatory postsynapses. BRAG2 interacts with AMPA receptors (Fig. 1) and can be co-immunoprecipitated with GluA2 from neurons (Fig. 1E). Subcellular fractionation demonstrates an enrichment of BRAG2 in the PSD fractions, similarly to PSD-95 (Fig. 2B, C). BRAG2 mediated activation of Arf6 is necessary for the internalization of AMPA receptors during chemical (mGluR-mediated; Fig.6, 7) and electrical (NMDAR-mediated; Fig.8) LTD at Schaffer Collateral CA3-CA1 synapses in acute hippocampal brain slices. Knock-down using BRAG2-RNAi and Knock-out (KO) using Cre-infected conditional BRAG2 KO mice prevents the expression of LTD. In summary, BRAG2 is essential for the expression of hippocampal LTD, which is mediated by the endocytosis of AMPA receptors. The specificity of the BRAG2 antibody was demonstrated by Western Blot using KD and KO controls (Fig. S3 and S4). 3/7/2017 Pim Fig.3 interaction of Brag2 with the AMPAR subunit regulats its GEF activity towards Arf6. Fig.5 "BRAG2 Mediates mGluR-Induced Arf6 Activation" Fig.7 "BRAG2-Mediated Arf6 Activation Is an Essential Step in mGluR-Induced AMPAR Internalization" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1060 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |