| Annotated protein: | IQ motif and SEC7 domain-containing protein 1. Gene symbol: IQSEC1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R0S2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IQSEC1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | retina, glutamatergic |
| Annotated paper: | Sakagami H, et al. "Distinct synaptic localization patterns of brefeldin A-resistant guanine nucleotide exchange factors BRAG2 and BRAG3 in the mouse retina" J Comp Neurol. 2013 Mar 1;521(4):860-76 PMID:22886754 |
| Figure(s): | Fig.1, 5, 7 |
| Annotation description: | GEP100/BRAG2/IQSEC1 localizes to glutamatergic postsynaptic specializations in the inner plexiform layer of the mouse retina. Immunohistochemical analysis demonstrated a strong co-localization of the immunoreactivity of BRAG2 with PSD-95 (Fig. 5A-E) and AMPA receptors (Fig. 5K-O), but not with gephrin, a marker of inhibitory postsynapses (Fig. 5F-J). The presence of BRAG2 at excitatory postsynapses was confirmed by pre-embedding immuno EM, showing that BRAG2 localizes to AMPA-containing postsynaptic processes receiving input from glutamatergic bipolar ribbon synapses in the IPL (Fig. 7). The guinea pig antibody against BRAG2 was generated in the study and tested for its specificity by Western Blot and by ICC after expression of the protein in COS-7 cells (Fig. 1). The antibody detects three BRAG2 isoforms or slice variants, but exhibits no cross-reactivity with BRAG1 or -3. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1058 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |