| Annotated protein: | IQ motif and SEC7 domain-containing protein 3. Gene symbol: IQSEC3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3TES0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IQSEC3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization of symmetric synapse (GO:0099629) |
| Synapse type(s): | cerebellum, GABAergic spinal cord, glycinergic hippocampus, GABAergic |
| Annotated paper: | Fukaya M, et al. "SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses" J Neurochem. 2011 Mar;116(6):1122-37 PMID:21198641 |
| Figure(s): | Fig. 3, 4, 5 |
| Annotation description: | SynArfGEF/BRAG3/IQSEC3 localizes to postsynaptic specializations of symmetrical, inhibitory synapses. Immunohistochemical analysis of the mouse forebrain, cerebellum, and spinal cord revealed that IQSEC3 colocalizes with gephrin, a component of inhibitory postsynaptic specializations (Fig. 1e-g, o-q; s-u; Fig.4b-d, k-n), but not with PSD-95, a component of the postsynaptic density of asymmetric, excitatory synapses (Fig.1h-i). Further, IQSEC3 immunoreactivity colocalizes with the staining pattern of GABAARs (Fig.4e-g) and GlyRs (Fig.4k-n), but not of GluA2Rs (Fig.h-j). Pre-embedding immuno-EM confirmed the presence of IQSEC3-immunoreactivity at postsynaptic specializations of symmetrical synapses (Fig.5). Antibodies (rabbit and guinea pig) were generated in this study using a GST-IQSEC3 fusion protein and specificity was tested by Western Blot (Fig.2) and for IHC by pre-absorption of the antigen (Fig.3b). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Characterization of the interaction of IQSEC3 and gephrin. @ PMID:27369185 |
| SynGO annotation ID: | 1051 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |